101 Marco Alésio Figueiredo Pereira, Tatiana Moraes da Silva Heck, Fabiano Costa de Oliveira, Rodrigo Staggemeier, Daniela Müller de Quevedo e Sabrina Esteves de Matos Almeida 2.3 Viral concentration, recovery and quantification on the surface of the water depth and edge sediments The water concentration for viral analysis was performed by ultracentrifugation, described by Ichim and Wells (2011). The viral genomes present in the samples were extracted through the MINI SPIN PLUS extraction kit (BIOPUR®). Molecular detection occurred through the technique of quantitative Polymerase Chain Reaction (qPCR), using the DNA intercalant Syber Green, using primers specific for HAdV-C and HAdV-F according to Wolf et al. (2010), and BAdV according to Dalla Vecchia et al. (2015). For each reaction, negative and positive controls were used and the technique sensitivity was 40 to 60 copies per reaction. 2.4 Hydrometric data The data of antecedent precipitation, flow and average water temperature come frommonitoring stations located in the watershed (Table 2). These data were obtained from National Water and Basic Sanitation Agency (ANA), a federal agency responsible for the implementation of Brazilian water resources management, in website in the Hydrological Information System (HidroWeb) section at: http:// www.snirh.gov.br/. The flow hydrometric station (Table 1) were determined from river level on the day of the field collections, by using the equation of the key-curve of each station. The flows in the sites where the AdV collections were carried out were determined applying the hydrological regionalization methodology, equation 1 and 2, described by Chaves et al. (2002). Hydrometric stations can be seen in Figure 1. (1) (2) where (m³.s-¹) is the flow rate at the location of interest (z), upstream (m), and downstream (j), respectively. (km²) are the respective drainage areas of each section.
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